Recent studies indicate that extracts of garlic could be
used to beat multi-drug resistant (MDR) and extremely drug resistant
(XDR) tuberculosis and other antibiotic resistant diseases like cystic
fibrosis.
RESEARCHERS have confirmed that garlic could be used to treat multi-drug resistant (MDR) and extremely drug resistant (XDR) tuberculosis and other antibiotic resistant diseases like cystic fibrosis (a disease passed down through families that causes thick, sticky mucus to build up in the lungs, digestive tract, and
other areas of the body).
Saudi and Pakistani researchers in a study titled: “Anti-mycobacterial activity of garlic (Allium sativum) against multi-drug resistant and non-multi-drug resistant Mycobacterium tuberculosis (MTB)” concluded, “this study demonstrated that the garlic extract has showed its effectiveness against clinical isolates of MDR M. tuberculosis. It is worthwhile to utilise garlic as natural supplement with other standard anti tuberculosis treatment (ATT). It is corresponding that substitute medicines practices with plant extracts including garlic as a means of decreasing the burden of drug resistance and reducing the cost of management of diseases would be of public health importance.”
The researchers in the study Pakistan Journal of Pharmaceutical Science investigated a total of 20 clinical isolates of MTB including 15 MDR and five non-MDR strains. Ethanolic extract of garlic was prepared by maceration method. Minimum inhibitory concentration (MIC) was performed by using 7H9 middle brook broth dilution technique. MIC of garlic extract was ranged from one to three mg/ml, showing inhibitory effects of garlic against both non-MDR and MDR M tuberculosis isolates.
According to the researchers, “alternate medicine practices with plant extracts including garlic should be considered to decrease the burden of drug resistance and cost in the management of diseases. The use of garlic against MDR-TB may be of great importance regarding public health.”
In another study published in The Internet Journal of Infectious Diseases, antibacterial activity of A. sativum was tested against gram-positive and gram-negative bacterial isolates from Urinary Tract of Indian patients, which were confirmed for resistant against commonly used antibiotics for urinary tract infections.
In present study titled: “Antibacterial activity of allicin from Allium sativum against antibiotic resistant uropathogens,” only five quantities (10, 20, 30, 40 and 50µg) of aqueous allicin from A. sativum cloves and leaves were used, which has antibacterial activity against test isolates by disc diffusion method. The maximum inhibitory activity of allicin against all test isolates was observed at 40µg and the quantity was found statistically significant for antibacterial activity of allicin extracted from A. sativum cloves and leaves against Urino-genital Tract (UT) bacterial isolates.
Meanwhile, Danish researchers and collaborators have pinpointed a constituent of garlic that attacks a key step in development of biofilms, in an effort they hope may offer help in particular for patients with cystic fibrosis.
A biofilm is a complex aggregation of microorganisms growing on a solid substrate. Bacterial biofilms are far more resistant than individual bacteria to the armories of antibiotics that have devised to combat them.
The research is published in the May 2012 issue of Antimicrobial Agents and Chemotherapy.
In earlier work, Givskov and his colleagues showed that “crude extracts of garlic inhibit the expression of a large number of genes that are controlled by bacterial quorum sensing (communication among bacterial cells involved in biofilm development), and that extracts promote a rapid clearing of pulmonary Pseudomonas aeruginosa infection in mice.” “These findings encouraged us to identify and assess the efficacy of the pure active compound,” he said.
Jakobsen said that compound turned out to be ajoene, the major constituent of a multitude of sulphur-containing compounds produced when garlic is crushed. The team then showed in P. aeruginosa that ajoene inhibits expression of 11 genes that are controlled by quorum sensing. “These key genes are regarded as crucial for the ability of P. aeruginosa to cause disease,” he said.
Pseudomonas aeruginosa is a common bacterium that can cause disease in animals, including humans. Jakobsen further explained, “we also found ajoene to reduce the production of rhamnolipid, a compound that shields the biofilm bacteria from the white blood cells that otherwise would destroy bacteria, and that by combining ajoene with the antibiotic tobramycin, it was possible to kill over 90 per cent of bacteria living in a biofilm.
“Our study is part of a series of comprehensive investigations of natural compounds targeting bacterial quorum sensing systems, and it further strengthens previous proof of concept research we conducted on the potential of compounds which block communication among pathogen cells in contrast to simply killing bacteria, as conventional antibiotics do,” says Jakobsen. Such alternative approaches “may postpone or minimise development of antibiotic resistance.”
Share your thoughts...thanks!
RESEARCHERS have confirmed that garlic could be used to treat multi-drug resistant (MDR) and extremely drug resistant (XDR) tuberculosis and other antibiotic resistant diseases like cystic fibrosis (a disease passed down through families that causes thick, sticky mucus to build up in the lungs, digestive tract, and
other areas of the body).
Saudi and Pakistani researchers in a study titled: “Anti-mycobacterial activity of garlic (Allium sativum) against multi-drug resistant and non-multi-drug resistant Mycobacterium tuberculosis (MTB)” concluded, “this study demonstrated that the garlic extract has showed its effectiveness against clinical isolates of MDR M. tuberculosis. It is worthwhile to utilise garlic as natural supplement with other standard anti tuberculosis treatment (ATT). It is corresponding that substitute medicines practices with plant extracts including garlic as a means of decreasing the burden of drug resistance and reducing the cost of management of diseases would be of public health importance.”
The researchers in the study Pakistan Journal of Pharmaceutical Science investigated a total of 20 clinical isolates of MTB including 15 MDR and five non-MDR strains. Ethanolic extract of garlic was prepared by maceration method. Minimum inhibitory concentration (MIC) was performed by using 7H9 middle brook broth dilution technique. MIC of garlic extract was ranged from one to three mg/ml, showing inhibitory effects of garlic against both non-MDR and MDR M tuberculosis isolates.
According to the researchers, “alternate medicine practices with plant extracts including garlic should be considered to decrease the burden of drug resistance and cost in the management of diseases. The use of garlic against MDR-TB may be of great importance regarding public health.”
In another study published in The Internet Journal of Infectious Diseases, antibacterial activity of A. sativum was tested against gram-positive and gram-negative bacterial isolates from Urinary Tract of Indian patients, which were confirmed for resistant against commonly used antibiotics for urinary tract infections.
In present study titled: “Antibacterial activity of allicin from Allium sativum against antibiotic resistant uropathogens,” only five quantities (10, 20, 30, 40 and 50µg) of aqueous allicin from A. sativum cloves and leaves were used, which has antibacterial activity against test isolates by disc diffusion method. The maximum inhibitory activity of allicin against all test isolates was observed at 40µg and the quantity was found statistically significant for antibacterial activity of allicin extracted from A. sativum cloves and leaves against Urino-genital Tract (UT) bacterial isolates.
Meanwhile, Danish researchers and collaborators have pinpointed a constituent of garlic that attacks a key step in development of biofilms, in an effort they hope may offer help in particular for patients with cystic fibrosis.
A biofilm is a complex aggregation of microorganisms growing on a solid substrate. Bacterial biofilms are far more resistant than individual bacteria to the armories of antibiotics that have devised to combat them.
The research is published in the May 2012 issue of Antimicrobial Agents and Chemotherapy.
In earlier work, Givskov and his colleagues showed that “crude extracts of garlic inhibit the expression of a large number of genes that are controlled by bacterial quorum sensing (communication among bacterial cells involved in biofilm development), and that extracts promote a rapid clearing of pulmonary Pseudomonas aeruginosa infection in mice.” “These findings encouraged us to identify and assess the efficacy of the pure active compound,” he said.
Jakobsen said that compound turned out to be ajoene, the major constituent of a multitude of sulphur-containing compounds produced when garlic is crushed. The team then showed in P. aeruginosa that ajoene inhibits expression of 11 genes that are controlled by quorum sensing. “These key genes are regarded as crucial for the ability of P. aeruginosa to cause disease,” he said.
Pseudomonas aeruginosa is a common bacterium that can cause disease in animals, including humans. Jakobsen further explained, “we also found ajoene to reduce the production of rhamnolipid, a compound that shields the biofilm bacteria from the white blood cells that otherwise would destroy bacteria, and that by combining ajoene with the antibiotic tobramycin, it was possible to kill over 90 per cent of bacteria living in a biofilm.
“Our study is part of a series of comprehensive investigations of natural compounds targeting bacterial quorum sensing systems, and it further strengthens previous proof of concept research we conducted on the potential of compounds which block communication among pathogen cells in contrast to simply killing bacteria, as conventional antibiotics do,” says Jakobsen. Such alternative approaches “may postpone or minimise development of antibiotic resistance.”
Share your thoughts...thanks!
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